Insertion junction: LMJ.RY0402.072939_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.072939
Chromosome:chromosome_13
Location:2255549
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g578750 TBA1 PsbA translation factor antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCACAAGCAATGCGTGCAGTGCAGTGCGAC
Internal bar code:GGGTAGACTGTGGGAAGATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:625
LEAP-Seq percent confirming:93.5435
LEAP-Seq n confirming:1246
LEAP-Seq n nonconfirming:86
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAATCCGTCAAAACCTAA
Suggested primer 2:AGTGCAGGTAGATGCGTGTG