| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.072939 |
| Chromosome: | chromosome 13 |
| Location: | 2255549 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578750 | TBA1 | (1 of 1) K00207 - dihydropyrimidine dehydrogenase (NADP+) (DPYD); Translation factor for chloroplast psbA mRNA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAAGCAATGCGTGCAGTGCAGTGCGAC |
| Internal bar code: | GGGTAGACTGTGGGAAGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 93.5435 |
| LEAP-Seq n confirming: | 1246 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAATCCGTCAAAACCTAA |
| Suggested primer 2: | AGTGCAGGTAGATGCGTGTG |