Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.072939 |
Chromosome: | chromosome_13 |
Location: | 2255549 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g578750 | TBA1 | PsbA translation factor | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACAAGCAATGCGTGCAGTGCAGTGCGAC |
Internal bar code: | GGGTAGACTGTGGGAAGATGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 625 |
LEAP-Seq percent confirming: | 93.5435 |
LEAP-Seq n confirming: | 1246 |
LEAP-Seq n nonconfirming: | 86 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCAATCCGTCAAAACCTAA |
Suggested primer 2: | AGTGCAGGTAGATGCGTGTG |