| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.072956 |
| Chromosome: | chromosome 17 |
| Location: | 1271917 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705450 | LCI26 | Low-CO2-induced U-box protein; (1 of 1) PTHR22849//PTHR22849:SF45 - WDSAM1 PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCGCCCTGAATGCAAAAGAAAGCAA |
| Internal bar code: | CGTTGTTCGTAAGCTCGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 97.5556 |
| LEAP-Seq n confirming: | 439 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCTACACAACCACAGG |
| Suggested primer 2: | GGTTACGAATAGGACGGCAA |