| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073052 |
| Chromosome: | chromosome_1 |
| Location: | 7096464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g051250 | DLC4,DLE1 | Flagellar outer dynein arm 18 kDa light chain LC4 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGCAGGAGCGGAACGTGTGAGGCCTGTCA |
| Internal bar code: | CCTGTATACCCATTGTCAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 99.2547 |
| LEAP-Seq n confirming: | 1598 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAAGCCATGCAAGTGAC |
| Suggested primer 2: | GTACACAAGTGCCTCCGGTT |