| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073319 |
| Chromosome: | chromosome 12 |
| Location: | 9068536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542950 | CGL137,NUP54 | Nucleoporin 54; (1 of 1) K14308 - nuclear pore complex protein Nup54 (NUP54, NUP57) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCCTGCCTTGTTCCCGCTACCACACAG |
| Internal bar code: | GAAGAGTCAGGGAGGATTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGTATTGAACGGGGTG |
| Suggested primer 2: | CTGAATCTGGATGCAGGTGA |