| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074989 |
| Chromosome: | chromosome 12 |
| Location: | 1683688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485800 | FTSH1,FHL1 | FtsH-like membrane ATPase/metalloprotease; (1 of 1) PTHR23076//PTHR23076:SF33 - METALLOPROTEASE M41 FTSH // ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 1, CHLOROPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATGGAGCTTAGGGGCTAGCATGGCCCT |
| Internal bar code: | ATCTCGCGTAGCTTTTTCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 98.1308 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAGCTGTAGAGGGGACG |
| Suggested primer 2: | ACCCAACACAAGAGCGTACC |