| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075010 |
| Chromosome: | chromosome_3 |
| Location: | 4559056 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g176833 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCTCACGGAGGCGCCGCGAAACCCCAAG |
| Internal bar code: | ATCGACGACTCCTGGGGATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 97.6691 |
| LEAP-Seq n confirming: | 5112 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGCATCGCAAGTAAAC |
| Suggested primer 2: | ACGGGCGAGATGTGATAGTC |