| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075070 |
| Chromosome: | chromosome 12 |
| Location: | 5259099 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528400 | (1 of 1) PTHR22938:SF0 - ZINC FINGER PROTEIN 598 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCTGCGTTGAAGGTTACCGACTCCAAC |
| Internal bar code: | GACTGTTGCGCCTACTCATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 96.9925 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAAGGAGCTCGAGGGTG |
| Suggested primer 2: | CCTTCAGGCTGTCAGACACA |