| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075207 |
| Chromosome: | chromosome 7 |
| Location: | 418861 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315100 | (1 of 2) 1.5.4.1 - Pyrimidodiazepine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGCAAAGCCGGGATGAAGTCATGGCC |
| Internal bar code: | GGATGATTTCAGGGACCACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 97.3349 |
| LEAP-Seq n confirming: | 2447 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTGCTGAGGCTTCATT |
| Suggested primer 2: | GTGGTTCTTGCAGTTGCTGA |