Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.075388 |
Chromosome: | chromosome_12 |
Location: | 8335027 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g549550 | RSP19,TUB2 | Beta tubulin 2 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCAACGAGGCCACCGGTGGTGAGTTGCCT |
Internal bar code: | GTATTTCCGTACAAATATATTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 193 |
LEAP-Seq percent confirming: | 99.5425 |
LEAP-Seq n confirming: | 1088 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGAGTGGCAGACCTGGAAG |
Suggested primer 2: | AGCGAGCTACCAAAGCCATA |