| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075561 |
| Chromosome: | chromosome 12 |
| Location: | 5926832 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCATGGCGCCTAGGGCCCGGGGGCGGG |
| Internal bar code: | CGAAGGTCGTATTAGCCGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 98.756 |
| LEAP-Seq n confirming: | 2064 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAACTAGAGGGCCGAGAC |
| Suggested primer 2: | GGGATTAGTGTCCACACGCT |