| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076008 |
| Chromosome: | chromosome 16 |
| Location: | 2977798 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664301 | POLQ1 | DNA polymerase theta; (1 of 2) K02349 - DNA polymerase theta [EC:2.7.7.7] (POLQ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGCGCCCCACACCCACCCCACAAGCGT |
| Internal bar code: | AGTCCTAATGATACAATCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 99.5154 |
| LEAP-Seq n confirming: | 1232 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCAACTTCCTGCTGCCT |
| Suggested primer 2: | AACACCATGTAGCTGAGGGG |