Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.076426 |
Chromosome: | chromosome_12 |
Location: | 1361083 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g488450 | VSP3 | Extracellular matrix protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCTCTCGAACCGCTACTACTCGTACTGGT |
Internal bar code: | GACACGGTGGGCAACATTGTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 664 |
LEAP-Seq percent confirming: | 84.6354 |
LEAP-Seq n confirming: | 2275 |
LEAP-Seq n nonconfirming: | 413 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTGTCAGACATCAGCAGT |
Suggested primer 2: | CACCGCTCATGTCTTCGTAA |