| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076702 |
| Chromosome: | chromosome 12 |
| Location: | 5090564 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526701 | (1 of 7) PTHR10110 - SODIUM/HYDROGEN EXCHANGER | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGGCACGCAGCTGCACGCAGCCACAAT |
| Internal bar code: | CCCGTGATCTGGTTTGAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 926 |
| LEAP-Seq percent confirming: | 98.7563 |
| LEAP-Seq n confirming: | 2144 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTTGGGTCAAGTGAGGT |
| Suggested primer 2: | CCCACACACAAACACACACA |