| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077214 |
| Chromosome: | chromosome 7 |
| Location: | 2482247 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329300 | MSC1 | Mechanosensitive ion channel; (1 of 1) PTHR30221 - SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGCACCCATGTGCGCATCTCCTCACT |
| Internal bar code: | CCACCGGCGGTGGTCGGATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.3254 |
| LEAP-Seq n confirming: | 3681 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGAAAGCTCTGTGTCA |
| Suggested primer 2: | GAAGTAATAAGCGCGCCAAG |