| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077628 |
| Chromosome: | chromosome 6 |
| Location: | 8137989 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305000 | Conserved WD40-repeat Protein; (1 of 1) PTHR22850//PTHR22850:SF87 - WD40 REPEAT FAMILY // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTTCGGGTACCTCTCACTGACCATGC |
| Internal bar code: | TTGTTCTCTAGCGGAGTCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 52 |
| LEAP-Seq percent confirming: | 99.1329 |
| LEAP-Seq n confirming: | 343 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTCGTTTTTGGGAAAC |
| Suggested primer 2: | GGACAACCGAGTCCTTGTGT |