| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078508 |
| Chromosome: | chromosome 2 |
| Location: | 5887990 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111450 | TEF4 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese); Rhodanese-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGATTCATTGGGTGTTGATAAAATGGG |
| Internal bar code: | GCTGACTTGCGGGTGTAGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 98.6325 |
| LEAP-Seq n confirming: | 1731 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGCTGTCGCTTTTGTT |
| Suggested primer 2: | TCGCGTTGAACTCTTTCCTT |