Insertion junction: LMJ.RY0402.078934_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.078934
Chromosome:chromosome 1
Location:487053
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCTGCCCACCCGGCATGGTCGGCACGG
Internal bar code:GTAGCACAAGGGAGGCGGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:290
LEAP-Seq percent confirming:96.4758
LEAP-Seq n confirming:219
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCAACTCCATCTTCAGC
Suggested primer 2:GTGCATAGGTTGCAGGGTTT