| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079184 |
| Chromosome: | chromosome 13 |
| Location: | 1653437 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573900 | NSS3 | (1 of 1) PTHR11819//PTHR11819:SF159 - SODIUM/SOLUTE SYMPORTER // SUBFAMILY NOT NAMED; Sodium:solute symporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTCTTGCTGGTCATGGAGGTGCAGGGC |
| Internal bar code: | CGGGTTGTGTAGGTTTTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 98.6147 |
| LEAP-Seq n confirming: | 4983 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGACGAGAGCGATGTTGA |
| Suggested primer 2: | AGGATGCCCACAACTACTGG |