| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079341 |
| Chromosome: | chromosome 9 |
| Location: | 3827452 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391801 | VSP4 | (1 of 8) PTHR11339:SF290 - PROTEIN T26A8.1; Hydroxyproline-rich cell wall glycoprotein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTAGTTATCGTATGATGTTGTGTACG |
| Internal bar code: | TGAATTTCGCTCTGTAGCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 98.1857 |
| LEAP-Seq n confirming: | 920 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGGAGGAGTGAGGGCT |
| Suggested primer 2: | GTAGAAGAGCGGGTTGCTTG |