| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079442 |
| Chromosome: | chromosome 3 |
| Location: | 6315035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193400 | (1 of 2) PTHR30603 - RNA POLYMERASE SIGMA FACTOR RPO | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCGTAGTCCACAGACCTTAGTAGCACT |
| Internal bar code: | AGTGCGATGCCTGCGAAGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 98.6278 |
| LEAP-Seq n confirming: | 575 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCAGCAGAATGTATGCG |
| Suggested primer 2: | GTCAGGCTGCAGTTTGTTCA |