Insertion junction: LMJ.RY0402.079861_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.079861
Chromosome:chromosome 5
Location:426271
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g232751 (1 of 2) PTHR23518:SF2 - MULTIDRUG RESISTANCE PROTEIN MDTH CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTCAACATGCTGGCGCAGGTGTGCGCGC
Internal bar code:AGTCCAAGCAAGTACTGTGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:99.3333
LEAP-Seq n confirming:298
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACAATCACAGGGGTGGAGAG
Suggested primer 2:GGGCATGTAGGAGGAGATCA