| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079967 |
| Chromosome: | chromosome 16 |
| Location: | 5813109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678050 | SRS14,SRE3 | Pre-mRNA splicing factor, SR-related; (1 of 1) K13171 - serine/arginine repetitive matrix protein 1 (SRRM1, SRM160) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGCATTGGTGGCGGCACGACACAGGC |
| Internal bar code: | AACCCTGGGAAGTTGTTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 98.0305 |
| LEAP-Seq n confirming: | 1991 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATAAACAGCAACCCCAC |
| Suggested primer 2: | TCAACTGAGTCGATGTTCGC |