Insertion junction: LMJ.RY0402.080701_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080701
Chromosome:chromosome 13
Location:2219012
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGGGGGGGGGGCGGGAGGGAGGCCACA
Internal bar code:TGGATGTTCAAGGCTATAGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:99.4413
LEAP-Seq n confirming:178
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAAGAGTCGGTGGTGGGA
Suggested primer 2:AGGAGTGGGAGGAGGTGTTT