| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080926 |
| Chromosome: | chromosome 5 |
| Location: | 2510473 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235750 | MSH6 | DNA mismatch repair protein, MutS homolog; (1 of 1) K08737 - DNA mismatch repair protein MSH6 (MSH6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGCCACGTCAAAGCAGTGCCGACGTC |
| Internal bar code: | ATCGTTGTGACCGCTGTTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.8583 |
| LEAP-Seq n confirming: | 7047 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGAAACATACTTGCTGG |
| Suggested primer 2: | GGGCAAAGAGCTTCGTACAG |