| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080940 |
| Chromosome: | chromosome 5 |
| Location: | 446443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232550 | GPM4,PGM4,CA1P1 | (1 of 1) 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase; 2-Carboxy-D-arabinitol-1-phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGACCTGTGGCAGTACGGCATGCAG |
| Internal bar code: | GGTTAGCGGGGGGCAGCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.0934 |
| LEAP-Seq n confirming: | 1093 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACACTGCTGCTTCATTA |
| Suggested primer 2: | ACCACGTGTACGGTGTTGAA |