Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.081336 |
Chromosome: | chromosome_1 |
Location: | 843289 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g004350 | ALIX,HLM1,ROC77 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGAGAAGCTGATGCTCACACAGGCGCAGG |
Internal bar code: | GATGACGTCCGGGATCAAGACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 220 |
LEAP-Seq percent confirming: | 18.8362 |
LEAP-Seq n confirming: | 1761 |
LEAP-Seq n nonconfirming: | 7588 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGTTGGTTGTGATGAGTG |
Suggested primer 2: | CCATACGCACACTCATCCAC |