| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081386 |
| Chromosome: | chromosome 2 |
| Location: | 4167994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098300 | (1 of 1) K11547 - kinetochore protein NDC80 (NDC80, HEC1, TID3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCGCCACCCGCTGCCGCAGCTGCTCC |
| Internal bar code: | CGCAAGGTCGTCTGGTTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.0286 |
| LEAP-Seq n confirming: | 1835 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCTTCTCCACGTTGC |
| Suggested primer 2: | TGGACATGCAGAAGTTCGAG |