| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081626 |
| Chromosome: | chromosome 10 |
| Location: | 5193273 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456851 | TMG4 | tRNA (guanine-N1)-methyltransferase; (1 of 3) 2.1.1.221 - tRNA (guanine(9)-N(1))-methyltransferase / tRNA m(1)G(9)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCTGCTGGCAAGGCCGCCCCGCCTGGT |
| Internal bar code: | GGGGACATCGAACGCAGTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 96.9856 |
| LEAP-Seq n confirming: | 3507 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTTCCCTCTGCGTAATG |
| Suggested primer 2: | CCAGCTCAACTTGCTTTTCC |