| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081839 |
| Chromosome: | chromosome 6 |
| Location: | 2891472 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272200 | (1 of 3) PF08238 - Sel1 repeat (Sel1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGCTGGAACTGCTTATGACAATTGGCC |
| Internal bar code: | AGGGCTATCTTGCGCAAGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 99.7014 |
| LEAP-Seq n confirming: | 7012 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCATATGAGCAGTCGG |
| Suggested primer 2: | ATGTATCTGTACGGCCAGGG |