| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081975 |
| Chromosome: | chromosome 11 |
| Location: | 388693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467579 | CSC6 | Chlamydomonadale-specific family C protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTGTACTAACAAAAACACGGACAAAC |
| Internal bar code: | GAGAGGAGAGAAGTGACTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 97.5172 |
| LEAP-Seq n confirming: | 1414 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACTGCTAGATAGGCACC |
| Suggested primer 2: | GGTAGCACGTTGTCCGTTTT |