| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083560 |
| Chromosome: | chromosome 3 |
| Location: | 2467881 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159500 | ODC1 | (1 of 2) 4.1.1.17 - Ornithine decarboxylase / L-ornithine carboxy-lyase; Ornithine decarboxylase 1 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTAGTCCTGTGGCAATTTTAGCAGCGG |
| Internal bar code: | ATGTAAGTTTGGTATCCCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 98.4211 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGTCTACAAGGACGTGA |
| Suggested primer 2: | GCACGACTACGGGTAGGGTA |