| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083644 |
| Chromosome: | chromosome_4 |
| Location: | 674682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217951 | LCI33 | Low-CO2-inducible protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAAGACAGCCTCATGGAGCTCCGTTGGAG |
| Internal bar code: | GGTGCTGGATATCCCGAACCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 99.8024 |
| LEAP-Seq n confirming: | 3030 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTTCAAGCAAGGGCCTG |
| Suggested primer 2: | TGACAGCCTGGACAACAGAG |