Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.084323 |
Chromosome: | chromosome 7 |
Location: | 6200026 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g356350 | DXS1 | 1-deoxy-D-xylulose 5-phosphate synthase, chloroplast precursor | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGACTAGAATGAACAACGGGGAGATCC |
Internal bar code: | GCCGTTAGGGATGCGTATTTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 115 |
LEAP-Seq percent confirming: | 99.4118 |
LEAP-Seq n confirming: | 169 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGATCATTGGGAAGTCGCTG |
Suggested primer 2: | GGCTGCGGGATATACACACT |