Insertion junction: LMJ.RY0402.084323_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084323
Chromosome:chromosome 7
Location:6200026
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g356350 DXS1 1-deoxy-D-xylulose 5-phosphate synthase, chloroplast precursor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGACTAGAATGAACAACGGGGAGATCC
Internal bar code:GCCGTTAGGGATGCGTATTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:115
LEAP-Seq percent confirming:99.4118
LEAP-Seq n confirming:169
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGATCATTGGGAAGTCGCTG
Suggested primer 2:GGCTGCGGGATATACACACT