| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084509 |
| Chromosome: | chromosome 1 |
| Location: | 6799348 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049000 | PTD1,RAF2,CGL31 | (1 of 1) PTHR12599//PTHR12599:SF8 - PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE // SUBFAMILY NOT NAMED; RuBisCO assembly chaperone, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGGTAAAGGAGTGCAGGCTAGCACTG |
| Internal bar code: | GGTTGGCTCTTGGTTGATCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.5824 |
| LEAP-Seq n confirming: | 4292 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCATTGCTTGCTGGTC |
| Suggested primer 2: | CGTACTTCATCGCTCGAACA |