Insertion junction: LMJ.RY0402.085026_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.085026
Chromosome:chromosome 7
Location:1243983
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGCTGCAGTTGCGGTGCGACCGCTGCGA
Internal bar code:CCGGTCGGTCAAGTAACTCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:99.3377
LEAP-Seq n confirming:450
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGAGGCAGTGGTAGAGG
Suggested primer 2:GTCCCTCGTTCCTTTCCTTC