| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085051 |
| Chromosome: | chromosome 9 |
| Location: | 4039856 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393136 | (1 of 5) PF01417 - ENTH domain (ENTH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGAATTGCGATTCTACGTGACGTCGA |
| Internal bar code: | CCCTACGAGTGCAGGAGGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 98.8876 |
| LEAP-Seq n confirming: | 889 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGACGCCGCTCCTATTAC |
| Suggested primer 2: | AAGATGCAGGCATAGCGAGT |