| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085235 |
| Chromosome: | chromosome 12 |
| Location: | 2386800 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507300 | LCI30 | (1 of 1) K14842 - ribosome biogenesis protein NSA2 (NSA2); Low-CO2-inducible protein 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCATCAAGGAGCGGACGGCCCCCACCAA |
| Internal bar code: | AGGGTACAATTGCGAACCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 98.5611 |
| LEAP-Seq n confirming: | 411 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGAAGGTGGTGTTGGTGA |
| Suggested primer 2: | GACGAACACTCGAGAGAGGG |