| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085560 |
| Chromosome: | chromosome 16 |
| Location: | 7531606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688190 | (1 of 6) PTHR10527 - IMPORTIN BETA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCGCAGGCGCTGCTGCTGCGGCTGTG |
| Internal bar code: | TCTTTGAGGGTACGGCGCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 93.617 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCCTTGCTCCTCACCAG |
| Suggested primer 2: | GTTCTAGGCAAAAGCGATGC |