| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085608 |
| Chromosome: | chromosome 1 |
| Location: | 6704980 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048150 | HLA8 | (1 of 2) 3.1.31.1 - Micrococcal nuclease / Micrococcal endonuclease; Transcriptional co-activator/nuclease induced by high light | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCACCCACACACACACAGGCACAGGCA |
| Internal bar code: | GCGGGGCCTGAGACGGAGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 21.8645 |
| LEAP-Seq n confirming: | 1304 |
| LEAP-Seq n nonconfirming: | 4660 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGAAGAATTTCTCGCC |
| Suggested primer 2: | CGCGTTTTCTAATAGGAGCG |