| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086780 |
| Chromosome: | chromosome 5 |
| Location: | 3138232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239250 | (1 of 1) IPR003613//IPR003888//IPR003889//IPR013083 - U box domain // FY-rich, N-terminal // FY-rich, C-terminal // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAACGGAACTATGCCAATGCTCCCAAGA |
| Internal bar code: | TTTCCGCAAGTATTATTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 93.9216 |
| LEAP-Seq n confirming: | 479 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCACCAAGAAGTCGCTC |
| Suggested primer 2: | AAGGACCTTCTCGCTTCACA |