| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086990 |
| Chromosome: | chromosome 9 |
| Location: | 5951388 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402775 | VTC4,ROC81 | (1 of 1) PF02656//PF03105//PF09359 - Domain of unknown function (DUF202) (DUF202) // SPX domain (SPX) // VTC domain (VTC); Polyphosphate Polymerase activity | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGCATGATCAAGGAGAACCCCGAGGAC |
| Internal bar code: | GTGTCAGCAAGCGCTCCTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.5792 |
| LEAP-Seq n confirming: | 1893 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTGGAAAGACGCAGAG |
| Suggested primer 2: | TACACACCCCTCCTCCTCAC |