| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087057 |
| Chromosome: | chromosome 12 |
| Location: | 1130442 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490300 | MSD4 | (1 of 5) K04564 - superoxide dismutase, Fe-Mn family (SOD2); Mn superoxide dismutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGGCAGCTGCACGCCGCCGCGGCTGCA |
| Internal bar code: | GTTCGTTCGCTGAGGTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 99.7405 |
| LEAP-Seq n confirming: | 2306 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTTCCCAAAACACACAC |
| Suggested primer 2: | AGGCACTGCTGGATGACTTT |