| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087520 |
| Chromosome: | chromosome 10 |
| Location: | 2815220 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439350 | (1 of 3) PTHR17130:SF24 - GAN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCCCGTACGACGTGTGCGCATGCCCCC |
| Internal bar code: | GCGAAGGGAGAGCGAGGCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 98.9822 |
| LEAP-Seq n confirming: | 1945 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGGACTGAGCAAGTGA |
| Suggested primer 2: | GTCGAAGTTGGGCACGTAGT |