| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087613 |
| Chromosome: | chromosome 14 |
| Location: | 3697584 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631800 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGGCCCTCCGCGCCTCCAGTGGAGCAG |
| Internal bar code: | CGTGGCTCCTGTAGTAACTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.2136 |
| LEAP-Seq n confirming: | 757 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTCGGAATAAAATCCAT |
| Suggested primer 2: | GACGAGGATGAGGAGCAATC |