Insertion junction: LMJ.RY0402.087664_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.087664
Chromosome:chromosome 9
Location:1858766
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g395700 (1 of 5) PTHR18640//PTHR18640:SF9 - FAMILY NOT NAMED // SODIUM/METABOLITE COTRANSPORTER BASS4, CHLOROPLASTIC-RELATED; Sodium:bile acid symporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCGTGGCGCTTACACAGGCGCGTGACA
Internal bar code:GGCTTGTCGGCTTGACAGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:96.6983
LEAP-Seq n confirming:3866
LEAP-Seq n nonconfirming:132
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GAGAAGGTTGTGGACGTGGT
Suggested primer 2:TCCACCCAATCCATTCTCTC