| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087725 |
| Chromosome: | chromosome 9 |
| Location: | 4554546 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396139 | (1 of 5) K14326 - regulator of nonsense transcripts 1 (UPF1, RENT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTGCTTCCGGAGTTCCGGAACACCCGA |
| Internal bar code: | GCTGCCGGATGCCATGTACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1072 |
| LEAP-Seq percent confirming: | 89.7294 |
| LEAP-Seq n confirming: | 4045 |
| LEAP-Seq n nonconfirming: | 463 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCCATGTGTGTATGGCG |
| Suggested primer 2: | ATACAAGTGTTGCCAAGCCC |