| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088624 |
| Chromosome: | chromosome 17 |
| Location: | 3883406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728250 | (1 of 1) PF16908//PF16910 - Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Repeating coiled region of VPS13 (VPS13_mid_rpt) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACGACCGCCTGACCGCCGACGTCGCCA |
| Internal bar code: | GCCTATCAGGTGCCTCAACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 99.278 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGGAACAGGCTACACA |
| Suggested primer 2: | CTGCACTTCCCTCTCAGTCC |