| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088887 |
| Chromosome: | chromosome 1 |
| Location: | 5085931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035350 | (1 of 1) K07512 - mitochondrial trans-2-enoyl-CoA reductase (MECR, NRBF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGGCCGCCGCAAGGCCCTGGACGCCAT |
| Internal bar code: | TAGACTATGACGAGTGCATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 98.5075 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCCCAAAGTGGCTCAAC |
| Suggested primer 2: | CACACACACACACACACCGT |