Insertion junction: LMJ.RY0402.089551_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.089551
Chromosome:chromosome 13
Location:2219497
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTTTCCAACCGCCGCGCCGCTGCCCCT
Internal bar code:GCGCGCTGTGGGGGACTAGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:99.4924
LEAP-Seq n confirming:196
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGTGGGAGGAGGTGTTT
Suggested primer 2:GTGTGTGACGGAACAGATGG