| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089828 |
| Chromosome: | chromosome 12 |
| Location: | 7287518 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557700 | (1 of 1) K01069 - hydroxyacylglutathione hydrolase (E3.1.2.6, gloB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTAGGCGTGCTGTGTGCTTCAGGAGT |
| Internal bar code: | GTTAAGCAAAGTAATAGCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 81.4344 |
| LEAP-Seq n confirming: | 1601 |
| LEAP-Seq n nonconfirming: | 365 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCGACAAGTGTTCCCC |
| Suggested primer 2: | CGTTCTCCATATGAACGGCT |